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Home >> Antibodies >> Myosin Phosphatase Antibody / MYPT1 / PPP1R12A

Myosin Phosphatase Antibody / MYPT1 / PPP1R12A (F54070)

  Catalog No Formulation Size Price (USD)  
Image F54070-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F54070-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot testing of Myosin Phosphatase antibody at 1:4000: Lane 1) human HEK293, 2) (h) HeLa, 3) (h) HT-29, 4) (h) MCF-7, 5) mouse C2C12, 6) (m) NIH3T3 and 7) (m) uterus lysate. Predicted molecular weight ~115 kDa.
Western blot testing of Myosin Phosphatase antibody at 1:4000: Lane 1) human HEK293, 2) (h) HeLa, 3) (h) HT-29, 4) (h) MCF-7, 5) mouse NIH3T3 and 6) (m) C2C12 cell lysate. Predicted molecular weight ~115 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt O14974
Applications Western Blot : 1:4000
Limitations This Myosin Phosphatase antibody is available for research use only.
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Description

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the Myosin Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 481-514 from the human MYPT1 was used as the immunogen for the Myosin Phosphatase antibody.

Storage

Aliquot the Myosin Phosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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