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Home >> Antibodies >> Myosin 7 Antibody / MYH7

Myosin 7 Antibody / MYH7 [clone MYH7/727] (V4335)

  Catalog No Formulation Size Price (USD)  
Image V4335-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4335-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4335SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human breast carcinoma tissue with Myosin 7 antibody (clone MYH7/727). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name MYH7/727
Purity Protein A/G affinity
UniProt P12883
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This Myosin 7 antibody is available for research use only.
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Description

Myosin heavy chains are ubiquitous Actin-based motor proteins that convert the chemical energy derived from ATP hydrolysis into the mechanical energy that drives diverse motile processes in eukaryotic cells, including cytokinesis, vesicular transport and cellular locomotion. Muscle myosin is a heterohexamer consisting of two myosin heavy chains and two associated nonidentical pairs of myosin light chains. The seven myosin heavy chain isoforms that predominate in mammalian skeletal muscles include two developmental isoforms, MHC-embryonic (MYH3) and MHC-perinatal (MYH8); three adult skeletal muscle isoforms, MHC IIa (MYH2), MHC IIb (MYH4) and MHC IIx/d (MYH1); and MHC-beta/slow (MYH7 or MHC-beta), which is also expressed in cardiac muscle. Research indicates that mutations of the MYH7 gene causes hypertrophic cardiomyopathy.

Application Notes

Optimal dilution of the Myosin 7 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human protein was used as the immunogen for the Myosin 7 antibody.

Storage

Aliquot the Myosin 7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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