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Home >> Antibodies >> MYO6 Antibody / Unconventional Myosin-6

MYO6 Antibody / Unconventional Myosin-6 (RQ8294)

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  Catalog No Formulation Size Price (USD)  
Image RQ8294 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of fixed and permeabilized human HeLa cells with MYO6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MYO6 antibody.
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Flow cytometry testing of fixed and permeabilized human HeLa cells with MYO6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MYO6 antibody.
Western blot testing of 1) human A431 and 2) mouse brain tissue lysate with MYO6 antibody. Predicted molecular weight: 145-150 kDa (multiple isoforms).
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UM54
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This MYO6 antibody is available for research use only.
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Description

Unconventional myosin-VI, is a protein that in humans is coded for by MYO6. This gene encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The protein consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Application Notes

Optimal dilution of the MYO6 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (Q383-H786) was used as the immunogen for the MYO6 antibody.

Storage

After reconstitution, the MYO6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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