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Home >> Antibodies >> MGMT Antibody

MGMT Antibody (F43302)

  Catalog No Formulation Size Price (USD)  
Image F43302-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F43302-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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MGMT antibody western blot analysis in CEM lysate.
Confocal immunofluorescent analysis of MGMT antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P16455
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
Limitations This MGMT antibody is available for research use only.
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Description

MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Application Notes

Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for this MGMT antibody.

Storage

Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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