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Home >> Antibodies >> MCART1/2 Antibody / SLC25A51/52

MCART1/2 Antibody / SLC25A51/52 (RQ6906)

  Catalog No Formulation Size Price (USD)  
Image RQ6906 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MCART1/2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human serous adenocarcinoma of ovary tissue with MCART1/2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with MCART1/2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) monkey COS-7 and 3) mouse NIH 3T3 cell lysate with MCART1/2 antibody. Predicted molecular weight ~34 kDa.
Flow cytometry testing of human U-251 cells with MCART1/2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCART1/2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9H1U9 , Q3SY17
Localization Cytoplasmic (mitochondria)
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This MCART1/2 antibody is available for research use only.
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Description

SLC25A51 gene ontology annotations related to this gene include transmembrane transporter activity.

Application Notes

Optimal dilution of the MCART1/2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-E291) was used as the immunogen for the MCART1/2 antibody.

Storage

After reconstitution, the MCART1/2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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