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Home >> Antibodies >> LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF alpha

LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF alpha [clone rLEF1/8854] (V5056)

  Catalog No Formulation Size Price (USD)  
Image V5056-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5056-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5056SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil tissue with LEF1 antibody (clone rLEF1/8854). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with LEF1 antibody (clone rLEF1/8854). Inset: PBS instead of primary. Secondary antibody negative control.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rLEF1/8854
Purity Protein A/G affinity
UniProt Q9UJU2
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This LEF1 antibody is available for research use only.
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Description

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells.Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

Application Notes

Optimal dilution of the LEF1 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-399) from the human protein was used as the immunogen for the LEF1 antibody.

Storage

Aliquot the LEF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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