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Home >> Antibodies >> Krueppel-like factor 4 Antibody / KLF4

Krueppel-like factor 4 Antibody / KLF4 (RQ6883)

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  Catalog No Formulation Size Price (USD)  
Image RQ6883 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE rat stomach tissue with Krueppel-like factor 4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat stomach tissue with Krueppel-like factor 4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse stomach tissue with Krueppel-like factor 4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) PC-3 and 2) HeLa cell lysate with Krueppel-like factor 4 antibody. Predicted molecular weight: 50-60 kDa + possible ~75 kDa (phosphorylated form).
Flow cytometry testing of human HeLa cells with Krueppel-like factor 4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Krueppel-like factor 4 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O43474
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Krueppel-like factor 4 antibody is available for research use only.
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Description

Kruppel-like factor 4, also known as EZF or GKLF, is a protein that in humans is encoded by the KLF4 gene. This gene is mapped to 9q31.2. KLF4 gene encodes a member of the Kruppel family of transcription factors. This gene plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. It is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. This gene involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Application Notes

Optimal dilution of the Krueppel-like factor 4 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-H389) was used as the immunogen for the Krueppel-like factor 4 antibody.

Storage

After reconstitution, the Krueppel-like factor 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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