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Home >> Antibodies >> KIT Antibody

KIT Antibody [clone 566CT8.5.4] (F40394)

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  Catalog No Formulation Size Price (USD)  
Image F40394-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F40394-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Immunofluorescent analysis of U251 cells using KIT antibody. Ab was diluted at 1:100 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
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Immunofluorescent analysis of U251 cells using KIT antibody. Ab was diluted at 1:100 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Fluorescent confocal image of SY5Y cells stained with KIT antibody at 1:50. Note the highly specific localization of the immunosignal to the membranes, especially the plasma membrane.
KIT antibody western blot analysis in A549 lysate. Observed molecular weight: ~120/145kDa (precusor/mature).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgM
Clone Name 566CT8.5.4
Purity Purified
UniProt P10721
Applications Western blot : 1:100
Immunofluorescence : 1:10-1:100
Limitations This KIT antibody is available for research use only.
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Description

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Notes

Titration of the KIT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This KIT antibody was produced from mice immunized with human KIT recombinant protein.

Storage

Aliquot the KIT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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