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Home >> Antibodies >> KEPI Antibody / Kinase-enhanced PP1 inhibitor / PPP1R14C

KEPI Antibody / Kinase-enhanced PP1 inhibitor / PPP1R14C (RQ8239)

  Catalog No Formulation Size Price (USD)  
Image RQ8239 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of fixed and permeabilized human 293T cells with KEPI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KEPI antibody.
Western blot testing of 1) human 293T, 2) human K562, 3) rat H9C2(2-1) and 4) mouse Neuro-2a cell lysate with KEPI antibody. Predicted molecular weight ~18 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q8TAE6
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This KEPI antibody is available for research use only.
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Description

Protein phosphatase 1 regulatory subunit 14C, also called Kinase-enhanced PP1 inhibitor (KEPI), is an enzyme that in humans is encoded by the PPP1R14C gene. The degree of protein phosphorylation is regulated by a balance of protein kinase and phosphatase activities. Protein phosphatase-1 (PP1; see MIM 176875) is a signal-transducing phosphatase that influences neuronal activity, protein synthesis, metabolism, muscle contraction, and cell division. PPP1R14C is an inhibitor of PP1.

Application Notes

Optimal dilution of the KEPI antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids H67-V165) was used as the immunogen for the KEPI antibody.

Storage

After reconstitution, the KEPI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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