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Home >> Antibodies >> ISG15 Antibody

ISG15 Antibody (F41498)

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  Catalog No Formulation Size Price (USD)  
Image F41498-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F41498-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC analysis of FFPE human breast carcinoma tissue stained with the ISG15 antibody
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IHC analysis of FFPE human breast carcinoma tissue stained with the ISG15 antibody
Western blot analysis of ISG15 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (2). Expected molecular weight: 15-17 kDa.
ISG15 antibody western blot analysis in 293 lysate. Expected molecular weight: 15-17 kDa.
ISG15 antibody western blot analysis in Jurkat, K562 and AML-2 lysate treated with CQ (0/10/20/30uM) for 24 hours. (Courtesy of Biyin Cao, Soochow University, Center for Blood Research)
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P05161
Localization Cytoplasmic
Applications IHC (Paraffin) : 1:50-1:100
Western blot : 1:1000
Limitations This ISG15 antibody is available for research use only.
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Description

ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.

Application Notes

Titration of the ISG15 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 31-61 from the human protein was used as the immunogen for this ISG15 antibody.

Storage

Aliquot the ISG15 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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