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Home >> Antibodies >> IL1F6 Antibody / IL-36 alpha [Discontinued, view alternatives]

IL1F6 Antibody / IL-36 alpha [Discontinued, view alternatives] (RQ4152)

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  Catalog No Formulation Size Price (USD)  
Image RQ4152 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 299
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Western blot testing of human 1) Jurkat, 2) K562, 3) Raji, 4) 22RV1 and 5) mouse thymus lysate with IL1F6 antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa, routinely observed at 18-22 kDa.
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Western blot testing of human 1) Jurkat, 2) K562, 3) Raji, 4) 22RV1 and 5) mouse thymus lysate with IL1F6 antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa, routinely observed at 18-22 kDa.
IHC testing of FFPE mouse small intestine tissue with IL1F6 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse spleen tissue with IL1F6 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability Discontinued
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q9UHA7
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This IL1F6 antibody is available for research use only.
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Description

Interleukin-36 alpha also known as interleukin-1 family member 6 (IL1F6) is a protein that in humans is encoded by the IL36A gene. The protein encoded by this gene is a cytokine that can activate NF-kappa-B and MAPK signaling pathways to generate an inflammatory response. The encoded protein functions primarily in skin and demonstrates increased expression in psoriasis. In addition, decreased expression of this gene has been linked to a poor prognosis in both hepatocellular carcinoma and colorectal cancer patients.

Application Notes

Optimal dilution of the IL1F6 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein corresponding to amino acids K6-F158 was used as the immunogen for the IL1F6 antibody.

Storage

After reconstitution, the IL1F6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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