• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> IL-18 Antibody

IL-18 Antibody (R31447)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image R31447 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
IHC staining of FFPE rat spleen tissue with IL-18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
›
IHC staining of FFPE rat spleen tissue with IL-18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse spleen tissue with IL-18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse spleen tissue lysate with IL-18 antibody. Predicted molecular weight: 17~24 kDa.
Flow cytometry testing of fixed and permeabilized mouse HEPA1-6 cells with IL-18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL-18 antibody.
Availability 1-3 business days
Species Reactivity Rat, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.005% sodium azide
Gene ID 29197
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This IL-18 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : IHC-P
    Reactivity : Human
  • Applications : IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P, ELISA (protein)
    Reactivity : Mouse
  • Applications : WB
    Reactivity : Human
  • Applications : WB, IHC-P
    Reactivity : Human
  • Applications : WB
    Reactivity : Mouse
  • Applications : WB, ELISA (peptide)
    Reactivity : Human

Description

Interleukin-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL-18 is a cytokine that modulates both T helper type 1 (Th1) and Th2 responses. It is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the IL-18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Rat partial recombinant protein (AA 37-194) was used as the immunogen for this IL-18 antibody.

Storage

After reconstitution, the IL-18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all IL-18 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.