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Home >> Antibodies >> HSP75 Antibody / TRAP-1

HSP75 Antibody / TRAP-1 (RQ6811)

  Catalog No Formulation Size Price (USD)  
Image RQ6811 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human HL60 cells with HSP75 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSP75 antibody.
Western blot testing of 1) human K562, 2) human HL60, 3) human 293T, 4) human U-87 MG, 5) rat heart, 6) rat brain, 7) mouse heart and 8) mouse brain tissue lysate with HSP75 antibody. Expected molecular weight: 75-80 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q12931
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This HSP75 antibody is available for research use only.
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Description

Heat shock protein 75 kDa, mitochondrial is a protein that in humans is encoded by the TRAP1 gene. It is mapped to 16p13.3. This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. And this protein may function in regulating cellular stress responses. In addition, it was found that TRAP1 interacted with the N-terminal half of TNFR1. Also, TRAP1 interacted with the C-terminal ends of the proteins encoded by both multiple exostoses-causing genes, EXT1 and EXT2, but not with EXTL1 or EXTL3.

Application Notes

Optimal dilution of the HSP75 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids A618-H704) was used as the immunogen for the HSP75 antibody.

Storage

After reconstitution, the HSP75 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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