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Home >> Antibodies >> HP59 Antibody / Sialin / SLC17A5

HP59 Antibody / Sialin / SLC17A5 (RQ7660)

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  Catalog No Formulation Size Price (USD)  
Image RQ7660 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human thyroid cancer tissue with HP59 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human thyroid cancer tissue with HP59 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Jurkat, 2) human 293T, 3) monkey COS-7, 4) human ThP-1, 5) rat NRK and 6) mouse HBZY cell lysate with HP59 antibody. Predicted molecular weight ~55 kDa and 31 kDa (two isoform) which may be observed at higher molecular weights due to glycosylation.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9NRA2
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml;Direct ELISA
Limitations This HP59 antibody is available for research use only.
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Description

Sialin, also known as H(+)/nitrate cotransporter and H(+)/sialic acid cotransporter, is a protein which in humans is encoded by the SLC17A5 gene. This gene encodes a membrane transporter that exports free sialic acids that have been cleaved off of cell surface lipids and proteins from lysosomes. Mutations in this gene cause sialic acid storage diseases, including infantile sialic acid storage disorder and and Salla disease, an adult form.

Application Notes

Optimal dilution of the HP59 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-R365) was used as the immunogen for the HP59 antibody.

Storage

After reconstitution, the HP59 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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