Immunofluorescent staining of FFPE human MCF7 cells with HMGN2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human appendicitis with HMGN2 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human tonsil tissue with HMGN2 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human esophagus squama cancer with HMGN2 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human placenta with HMGN2 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human stomach cancer with HMGN2 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) HeLa, 2) A549 and 3) U-87 MG cell lysate with HMGN2 antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
Flow cytometry testing of human Raji cells with HMGN2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HMGN2 antibody.
Western blot testing of human 1) SH-SY5Y and 2) HL60 cell lysate with HMGN2 antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
IHC staining of FFPE human tonsil tissue with HMGN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with HMGN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with HMGN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with HMGN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.