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Home >> Antibodies >> HLA-G Antibody

HLA-G Antibody [clone HLAG/6686] (V8991)

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  Catalog No Formulation Size Price (USD)  
Image V8991-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8991-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8991SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human tonsil tissue with HLA-G antibody (clone HLAG/6686) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with HLA-G antibody (clone HLAG/6686) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate tissue with HLA-G antibody (clone HLAG/6686) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji cell lysate using HLA-G antibody (clone HLAG/6686).
IHC staining of FFPE human tonsil tissue with HLA-G antibody (clone HLAG/6686) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name HLAG/6686
Purity Protein A/G affinity
UniProt P17693
Localization Cell surface
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This HLA-G antibody is available for research use only.
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Description

Major histocompatibility complex (MHC), human leukocyte antigen (HLA) molecules are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and a non-covalently associated protein, 2-microglobulin. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a noncovalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

Application Notes

Optimal dilution of the HLA-G antibody should be determined by the researcher.

Immunogen

Purified His-tagged HLA-G protein was used as the immunogen for the HLA-G antibody.

Storage

Aliquot the HLA-G antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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