HER2 Antibody / ErbB2 [clone HRB2/451] (V2108)

Immunofluorescent staining of PFA-fixed human MCF-7 cells with HER2 antibody (green) and Reddot nuclear stain (red).

Flow cytometry testing of human MCF-7 cells with HER2 antibody at 1ug/million cells; Red=isotype control, Blue= HER2 antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 antibody (clone HRB2/451). These results demonstrate the foremost specificity of the HRB2/451 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free HER2 antibody (clone HRB2/451) as confirmation of integrity and purity.

Flow cytometry testing of human SK-BR3 cells with HER2 antibody at 1ug/million cells; Red=isotype control, Blue= HER2 antibody.

Immunofluorescent staining of MeOH-fixed human MCF-7 cells with HER2 antibody (green) and Reddot nuclear stain (red).