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Home >> Antibodies >> Heme Oxygenase 1 Antibody / HO-1 / HMOX1

Heme Oxygenase 1 Antibody / HO-1 / HMOX1 (R31511)

  Catalog No Formulation Size Price (USD)  
Image R31511 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE mouse liver tissue with Heme Oxygenase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with Heme Oxygenase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat PC-12, 2) mouse spleen, 3) mouse RAW264.7 and 4) mouse NIH 3T3 cell lysate with Heme Oxygenase 1 antibody. Predicted molecular weight ~32 kDa.
Availability Discontinued
Species Reactivity Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
Gene ID 15368
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This Heme Oxygenase 1 antibody is available for research use only.
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Description

Heme oxygenase 1, also called HMOX1 and HO-1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HO-1 activity is induced by its substrate heme and by various nonheme substances.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Heme Oxygenase 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse partial recombinant protein (AA 2-261) was used as the immunogen for this Heme Oxygenase 1 antibody.

Storage

After reconstitution, the Heme Oxygenase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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