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Home >> Antibodies >> H3K9cr Antibody / Crotonyl-Histone H3 (Lys9)

H3K9cr Antibody / Crotonyl-Histone H3 (Lys9) [clone RM339] (R20362)

  Catalog No Formulation Size Price (USD)  
Image R20362-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Microvalidated Recrabbitmono
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Western blot testing using H3K9cr antibody. Anti-Histone H3 and anti-G6PDH were used as controls. A crotonylation inducing metabolite was used to increase the H3K9cr signal.
Dot blot showing H3K9cr antibody reacts specifically to Histone H3 crotonylated at Lys9, and cross-reactivity with different peptides.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM339
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Applications Western blot : 1ug/ml-5ug/ml
Dot blot : 0.5ug/ml-2ug/ml
Limitations This recombinant H3K9cr antibody is available for research use only.
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Description

This antibody reacts to Histone H3 crotonylated at Lysine 9 (K9cr), and does not cross-react with acetylated or butyrylated Lysine 9. No cross reactivity with other crotonylated Lysines in histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K9cr antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A crotonyl-peptide corresponding to Crotonyl-Histone H3 (Lys9) was used as the immunogen for the recombinant H3K9cr antibody.

Storage

Store the recombinant H3K9cr antibody at -20oC.

Cross
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