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Home >> Antibodies >> Glutamic-oxaloacetic transaminase 1 Antibody / GOT1

Glutamic-oxaloacetic transaminase 1 Antibody / GOT1 (RQ6423)

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  Catalog No Formulation Size Price (USD)  
Image RQ6423 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human T-47D cells with Glutamic-oxaloacetic transaminase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human T-47D cells with Glutamic-oxaloacetic transaminase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE mouse brain tissue with Glutamic-oxaloacetic transaminase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Glutamic-oxaloacetic transaminase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) placenta, 2) T-47D, 3) HepG2, 4) Caco-2, 5) HL60, 6) K562, 7) HeLa, 8) rat brain, 9) mouse brain and 10) mouse liver tissue lysate with Glutamic-oxaloacetic transaminase 1 antibody. Predicted molecular weight ~41 kDa.
Flow cytometry testing of human HepG2 cells with Glutamic-oxaloacetic transaminase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Aspartate Aminotransferase antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P17174
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Aspartate Aminotransferase antibody is available for research use only.
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Description

Aspartate aminotransferase, cytoplasmic is an enzyme that in humans is encoded by the GOT1 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Application Notes

Optimal dilution of the Glutamic-oxaloacetic transaminase 1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids S5-Q413) was used as the immunogen for the Glutamic-oxaloacetic transaminase 1 antibody.

Storage

After reconstitution, the Glutamic-oxaloacetic transaminase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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