- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
GCAT Antibody / Glycine acetyltransferase (F54934)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
F54934-0.4ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.4 ml | 399 | |
|
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F54934-0.08ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.08 ml | 185 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse |
| Format | Purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Purified |
| UniProt | O75600 |
| Localization | Cytoplasmic, nuclear |
| Applications | Western blot : 1:500-1:1000 Flow cytometry : 1:10-1:50 (1x10e6 cells) Immunohistochemistry (FFPE) : 1:50-1:100 |
| Limitations | This GCAT antibody is available for research use only. |
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The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.
The stated application concentrations are suggested starting points. Titration of the GCAT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 155-181 from the human protein was used as the immunogen for the GCAT antibody.
Aliquot the GCAT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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