G3BP1 Antibody | RQ6870 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ6870	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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IHC staining of FFPE human cervical cancer tissue with G3BP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymphoma tissue with G3BP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human stomach cancer tissue with G3BP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with G3BP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with G3BP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human adenocarcinoma of the right colon with G3BP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carcinoma with G3BP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer with G3BP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human Caco-2 cells with G3BP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) Jurkat, 2) 293T, 3) A549, 4) MDA-MB-453, 5) U-87 MG, 6) Caco-2 and 7) Raji cell lysate with G3BP1 antibody. Predicted molecular weight ~52 kDa but routinley observed at up to ~68 kDa.

Flow cytometry testing of human Jurkat cells with G3BP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= G3BP1 antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q13283
Localization	Cytoplasmic, nuclear
Applications	Western blot : 1-2ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence (FFPE) : 5ug/ml Flow cytometry : 1-3ug/million cells
Limitations	This G3BP1 antibody is available for research use only.
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Description

Ras GTPase-activating protein-binding protein 1 is an enzyme that in humans is encoded by the G3BP1 gene. This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Application Notes

Optimal dilution of the G3BP1 antibody should be determined by the researcher.

Immunogen

Amino acids EKPEPVLEETAPEDAQK from the human protein were used as the immunogen for the G3BP1 antibody.

Storage

After reconstitution, the G3BP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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G3BP1 Antibody (RQ6870)

Description

Application Notes

Immunogen

Storage

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Area of Research

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Protocols

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