Fibroblast Activation Protein Alpha Antibody / FAP [clone FAP/4853] (V9497)

IHC staining of FFPE human liver tissue with Fibroblast Activation Protein antibody (clone FAP/4853). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human placental tissue with Fibroblast Activation Protein Alpha antibody (clone FAP/4853). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Fibroblast Activation Protein Alpha antibody (clone FAP/4853) as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Fibroblast Activation Protein Alpha antibody (clone FAP/4853). These results demonstrate the foremost specificity of the FAP/4853 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.