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Home >> Antibodies >> FGF2 Antibody

FGF2 Antibody (R30108)

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  Catalog No Formulation Size Price (USD)  
Image R30108 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human SiHa cells with FGF2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human SiHa cells with FGF2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) SK-O-V3, 3) SiHa and 4) U87 MG cell lysate with FGF2 antibody. Predicted molecular weight: 17-31 kDa (multiple isoforms).
Flow cytometry testing of fixed and permeabilized human SiHa cells with FGF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGF2 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P09038
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml (human protein tested)
Limitations This FGF2 antibody is available for research use only.
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Description

FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. It belongs to the fibroblast growth factor (FGF) family. FGFs exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF1 and -2 are present in relatively high levels in CNS. FGF2 is expressed at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the FGF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 143-288) was used as the immunogen for this FGF2 antibody.

Storage

After reconstitution, the FGF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

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   2.   View all FGF2 antibodies

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