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Home >> Antibodies >> Ferroportin 1 Antibody / FPN / SLC40A1

Ferroportin 1 Antibody / FPN / SLC40A1 (RQ7663)

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  Catalog No Formulation Size Price (USD)  
Image RQ7663 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human ovarian cancer tissue with Ferroportin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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Immunofluorescent staining of FFPE human ovarian cancer tissue with Ferroportin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human prostate adenocarcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectum adenocarcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Ferroportin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human plasma, 2) rat liver, 3) mouse spleen and 4) mouse liver tissue lysate with Ferroportin 1 antibody. Predicted molecular weight: ~62 kDa, but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of human U-2 OS cells with Ferroportin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ferroportin 1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9NP59
Localization Cell membrane, cytoplasm
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Ferroportin 1 antibody is available for research use only.
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Description

Ferroportin-1, also known as solute carrier family 40 member 1 (SLC40A1) or iron-regulated transporter 1 (IREG1), is a protein that in humans is encoded by the SLC40A1 gene, and is part of the Ferroportin (Fpn) Family.

Application Notes

Optimal dilution of the Ferroportin 1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-V571) was used as the immunogen for the Ferroportin 1 antibody.

Storage

After reconstitution, the Ferroportin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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