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Home >> Antibodies >> EZH2 Antibody / Enhancer of zeste homolog 2

EZH2 Antibody / Enhancer of zeste homolog 2 [clone EZH2/7507] (V5234)

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  Catalog No Formulation Size Price (USD)  
Image V5234-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5234-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5234SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human renal cell carcinoma tissue with EZH2 antibody (clone EZH2/7507). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human renal cell carcinoma tissue with EZH2 antibody (clone EZH2/7507). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human HDLM-2 cell lysate with EZH2 antibody (clone EZH2/7507). Predicted molecular weight: 85-95 kDa.
SDS-PAGE analysis of purified, BSA-free EZH2 antibody (clone EZH2/7507) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name EZH2/7507
Purity Protein A/G affinity
UniProt Q15910
Localization Nucleus
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This EZH2 antibody is available for research use only.
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Description

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.

Application Notes

Optimal dilution of the EZH2 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 300-550) from the human protein was used as the immunogen for the EZH2 antibody.

Storage

Aliquot the EZH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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