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Home >> Antibodies >> ETF1 Antibody / eRF1

ETF1 Antibody / eRF1 [clone 3E5.] (RQ6232)

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Image RQ6232 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of rat 1) liver, 2) testis, 3) kidney, 4) PC-12, and mouse 5) liver, 6) testis, 7) kidney and 8) RAW264.7 cell lysate with ETF1 antibody. Predicted molecular weight ~49 kDa.
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Western blot testing of rat 1) liver, 2) testis, 3) kidney, 4) PC-12, and mouse 5) liver, 6) testis, 7) kidney and 8) RAW264.7 cell lysate with ETF1 antibody. Predicted molecular weight ~49 kDa.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) K562, 4) Raji and 5) HepG2 cell lysate with ETF1 antibody. Predicted molecular weight ~49 kDa.
Immunofluorescent staining of FFPE human A431 cells with ETF1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat pancreas with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human Caco-2 cells with ETF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETF1 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with ETF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETF1 antibody.
Flow cytometry testing of rat RH35 cells with ETF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETF1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 3E5.
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P62495
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This ETF1 antibody is available for research use only.
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Description

Eukaryotic translation termination factor 1 (eRF1), also known as TB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.

Application Notes

Optimal dilution of the ETF1 antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids D9-K342) was used as the immunogen for the ETF1 antibody.

Storage

After reconstitution, the ETF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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