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Home >> Antibodies >> ERAB Antibody / HSD17B10 (N-Terminal Region)

ERAB Antibody / HSD17B10 (N-Terminal Region) (F54127)

  Catalog No Formulation Size Price (USD)  
Image F54127-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F54127-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot testing of ERAB antibody at 1:8000: Lane 1) human HeLa, 2) HepG2, 3) Jurkat, 4) K562 and 5) SH-SY5Y cell lysate. Predicted molecular weight ~27 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q99714
Applications Western Blot : 1:8000
Limitations This ERAB antibody is available for research use only.
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Description

ERAB / HSD17B10 functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. Catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the ERAB antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 14-48 from human ERAB was used as the immunogen for the ERAB antibody.

Storage

Aliquot the ERAB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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