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Home >> Antibodies >> ER Antibody / Estrogen Receptor alpha

ER Antibody / Estrogen Receptor alpha [clone NR3Ga-3] (V7061)

  Catalog No Formulation Size Price (USD)  
Image V7061-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7061-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7061SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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FACS testing of human MCF-7 cells: Black=cells alone; Green=isotype control; Red=PE conjugated ER antibody.
IHC testing of human breast carcinoma stained with Estrogen receptor antibody (NR3Ga-3). FFPE testing requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTAfor 10-20 min and cooling at RT for 20 minutes.
Western blot testing of MCF-7 cell lysate with ER antibody (clone NR3Ga-3). Predicted molecular weight of ER alpha: ~66 kDa.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name NR3Ga-3
Purity Protein G affinity chromatography
Gene ID 2099
Localization Intracellular, Nuclear
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-2ug/million cells
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This ER antibody is available for research use only.
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Description

Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.

Application Notes

Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.

Immunogen

Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.

Storage

Store the ER antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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