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Home >> Antibodies >> ER Antibody

ER Antibody (F47454)

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  Catalog No Formulation Size Price (USD)  
Image F47454-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
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F47454-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of ER antibody pre-incubated without (Lane 1) and with (2) blocking peptide in K562 lysate. Predicted molecular weight: 65-70 kDa.
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Western blot analysis of ER antibody pre-incubated without (Lane 1) and with (2) blocking peptide in K562 lysate. Predicted molecular weight: 65-70 kDa.
Fluorescent confocal image of MCF-7 cell stained with ER antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Pig, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P03372
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
Limitations This ER antibody is available for research use only.
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Description

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1. [UniProt]

Application Notes

Titration of the ER antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 452-480 from the human protein was used as the immunogen for this ER antibody (isoform 1).

Storage

Aliquot the ER antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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