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Home >> Antibodies >> ER alpha Antibody

ER alpha Antibody [clone NR3Ga-2] (V3836)

  Catalog No Formulation Size Price (USD)  
Image V3836-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3836-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3836SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human MCF-7 lysate with Estrogen Receptor alpha antibody (clone NR3Ga-2). Expected molecular weight ~67 kDa.
IHC testing of human breast carcinoma stained with ER alpha antibody (clone NR3Ga-2). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name NR3Ga-2
Purity Protein G affinity chromatography
UniProt P03372
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This ER alpha antibody is available for research use only.
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Description

Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.

Application Notes

The optimal dilution of the ER alpha antibody for each application should be determined by the researcher.

Immunogen

Full length human protein was used as the immunogen for this ER alpha antibody.

Storage

Store the ER alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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