Emerin Antibody (R32593)

Western blot testing of 1) rat skeletal muscle, 2) mouse heart and 3) human HeLa lysate with Emerin antibody at 0.5ug/ml. Predicted molecular weight 29 kDa but routinely observed at ~34 kDa.

Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma with Emerin antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma with Emerin antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IF/ICC staining of FFPE human U-2 OS with Emerin antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human endometrial carcinoma with Emerin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human esophageal squamous cell carcinoma with Emerin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human colon cancer with Emerin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Flow cytometry testing of human U-2 OS cells with Emerin antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Emerin antibody.

Immunofluorescent staining of FFPE human lung cancer tissue with Emerin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 10-20 min and allow to cool before testing.