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Home >> Antibodies >> Elastase 3B Antibody

Elastase 3B Antibody [clone ELTS3B-2] (V7213)

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  Catalog No Formulation Size Price (USD)  
Image V7213-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7213-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7213SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE rat pancreas with Elastase 3B antibody (clone ELTS3B-2). FFPE staining requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE rat pancreas with Elastase 3B antibody (clone ELTS3B-2). FFPE staining requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE mouse pancreas with Elastase 3B antibody (clone ELTS3B-2). FFPE staining requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human pancreas with Elastase 3B antibody (clone ELTS3B-2). FFPE staining requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name ELTS3B-2
Purity Protein G
UniProt P08861
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Elastase 3B antibody is available for research use only.
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Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Application Notes

Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

Immunogen

A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

Storage

Store the Elastase 3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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