Western blot testing of human HeLa cell lysate with EGFR antibody. Expected molecular weight: 134-170 kDa depending on glycosylation level.
IHC staining of FFPE human stomach cancer with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat stomach tissue with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine tissue with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with EGFR antibody at 1:50 dilution (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human HeLa cells with EGFR antibody at 1:150 dilution (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human HeLa cells with EGFR antibody at 1:1500 dilution (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human A431, 3) human A549, 4) human U-87 MG, 5) rat liver and 6) mouse liver tissue lysate with EGFR antibody. Expected molecular weight: 134-170 kDa depending on glycosylation level.