Western blot testing of 1) rat liver, 2) rat kidney, 3) rat RH35, 4) mouse liver, 5) mouse kidney and 6) mouse HEPA(1-6) cell lysate with ECHS1 antibody. Predicted molecular weight ~31 kDa, commonly observed at 27-31 kDa.
Western blot testing of human 1) HepG2, 2) HeLa, 3) HT1080, 4) 293T, 5) MCF7, 6) U-87 MG, 7) Caco-2 and 8) PC-3 cell lysate with ECHS1 antibody. Predicted molecular weight ~31 kDa, commonly observed at 27-31 kDa.
IHC staining of FFPE human liver cancer tissue with ECHS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with ECHS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with ECHS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with ECHS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with ECHS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with ECHS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with ECHS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human intestinal cancer tissue with ECHS1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human lung cancer tissue with ECHS1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human Caco-2 cells with ECHS1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human 293T cells with ECHS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ECHS1 antibody.
Flow cytometry testing of human HeLa cells with ECHS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ECHS1 antibody.