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Home >> Antibodies >> E2F1 Antibody

E2F1 Antibody (F50542)

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  Catalog No Formulation Size Price (USD)  
Image F50542-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50542-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of E2F1 antibody at 1:1000 dilution. Lane 1: NIH3T3 lysate; 2: A431; Predicted molecular weight: 48-60 kDa.
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Western blot testing of E2F1 antibody at 1:1000 dilution. Lane 1: NIH3T3 lysate; 2: A431; Predicted molecular weight: 48-60 kDa.
Western blot testing of E2F1 antibody at 1:2000 dilution + HeLa lysate; Predicted molecular weight: 48-60 kDa.
Western blot analysis of E2F1 antibody pre-incubated without (lane 1) and with (2) blocking peptide in 293T lysate. Predicted molecular weight: 48-70 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q01094
Applications Western blot : 1:1000
Limitations This E2F1 antibody is available for research use only.
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Description

E2F1 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. [UniProt]

Application Notes

Titration of the E2F1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 342-371 from the human protein was used as the immunogen for this E2F1 antibody.

Storage

Aliquot the E2F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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