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Home >> Antibodies >> DNAJC6 Antibody

DNAJC6 Antibody (F55053)

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  Catalog No Formulation Size Price (USD)  
Image F55053-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F55053-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of 1) human K562, 2) human HL60 and 3) mouse brain lysate with DNAJC6 antibody. Predicted molecular weight ~100 kDa.
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Western blot testing of 1) human K562, 2) human HL60 and 3) mouse brain lysate with DNAJC6 antibody. Predicted molecular weight ~100 kDa.
Western blot testing of 1) human K562, 2) human SH-SY-5Y, 3) human U-87 MG, 4) human HL60, 5) mouse cerebellum, 6) mouse brain and 7) rat cerebellum lysate with DNAJC6 antibody. Predicted molecular weight ~100 kDa.
IHC testing of FFPE human brain tissue with DNAJC6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with DNAJC6 antibody; Blue=isotype control, Green= DNAJC6 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt O75061
Localization Cytoplasmic
Applications Western blot : 1:500-1:1000
Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:10-1:50
Limitations This DNAJC6 antibody is available for research use only.
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  • Applications : WB, IHC-P, FACS, IF, Direct ELISA
    Reactivity : Human, Mouse, Rat

Description

DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain

Application Notes

The stated application concentrations are suggested starting points. Titration of the DNAJC6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 254-281 from the human protein was used as the immunogen for the DNAJC6 antibody.

Storage

Aliquot the DNAJC6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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