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Home >> Antibodies >> deltaNp63 Antibody / p40

deltaNp63 Antibody / p40 [clone ZR8] (V8629)

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  Catalog No Formulation Size Price (USD)  
Image V8629-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8629-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8629SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human skin with deltaNp63 antibody (clone ZR8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human skin with deltaNp63 antibody (clone ZR8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin with deltaNp63 antibody (clone ZR8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of PFA-fixed human HeLa cells with deltaNp63 antibody (clone ZR8, green) and Phalloidin (red).
Flow cytometry testing of PFA-fixed human HeLa cells with deltaNp63 antibody (clone ZR8); Red=isotype control, Blue= deltaNp63 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name ZR8
Purity Protein A affinity chromatography
UniProt Q9H3D4
Localization Nuclear
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This deltaNp63 antibody is available for research use only.
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Description

p63 consists of two major isoforms-TAp63 and delta-Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation-competent TA domain with homology to p53, which regulates the expression of the growth-inhibitory genes. In contrast, DNp63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally-inactive delta-N domain, which antagonizes the activity of TAp63 and p53. The p40 (clone ZR8) antibody recognizes exclusively delta-Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma.

Application Notes

Optimal dilution of the deltaNp63 antibody should be determined by the researcher.

Immunogen

Amino acids ENNAQTQFSEPQY were used as the immunogen for the deltaNp63 antibody.

Storage

Store the deltaNp63 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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