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Home >> Antibodies >> DAP3 Antibody

DAP3 Antibody (R34882)

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  Catalog No Formulation Size Price (USD)  
Image R34882-100UG 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide 100 ug 429
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Western blot testing of HeLa lysate with DAP3 antibody at 3ug/ml. Predicted molecular weight: ~46/41/42 kDa (isoforms 1/2/3).
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Western blot testing of HeLa lysate with DAP3 antibody at 3ug/ml. Predicted molecular weight: ~46/41/42 kDa (isoforms 1/2/3).
Western blot of HEK293 lysate overexpressing DAP3 probed with DAP3 antibody (mock transfection in lane 1). Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
Western blot of HeLa lysate (control in left lane, post si-RNA-mediated DAP3 knock-down expresson in right lane) with DAP3 antibody at 1ug/ml. Level of knock-down relative to Actin expression level was determined by RT-PCR. Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
ICC staining of guanidinium thiocyanate-treated HeLa cells before (left) and after (right) si-RNA-mediated DAP3 knock-down using DAP3 antibody at 3ug/ml. Detection by DyLight 488 (green); nuclear DAPI stain (blue).
Immunofluorescent staining of human MCF7 cells with DAP3 antibody at 5ug/ml.
IHC testing of FFPE human brain cortex tissue with DAP3 antibody at 2.5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
IHC testing of FFPE human tonsil tissue with DAP3 antibody at 2.5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (goat origin)
Isotype Goat Ig
Purity Antigen affinity
UniProt P51398
Gene ID 7818
Localization Cytoplasmic
Applications Western blot : 3-6ug/ml
IF/ICC : 1-5ug/ml
IHC (FFPE) : 2.5-5ug/ml
ELISA (peptide) LOD : 1:16000
Limitations This DAP3 antibody is available for research use only.
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Description

Additional name(s) for this target protein: Death associated protein 3

Application Notes

Optimal dilution of the DAP3 antibody should be determined by the researcher.

1. This DAP3 antibody will detect isoforms 1, 2 and 3.

Immunogen

Amino acids NPSLLERHCAYL were used as the immunogen for this DAP3 antibody. This sequence is common to isoforms 1, 2 and 3.

Storage

Aliquot and store the DAP3 antibody at -20oC.

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