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Home >> Antibodies >> Cytokeratin 5 Antibody

Cytokeratin 5 Antibody (R32587)

  Catalog No Formulation Size Price (USD)  
Image R32587 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC testing of FFPE human tonsil tissue with Cytokeratin 5 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human esophageal SCC tissue with Cytokeratin 5 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A431, 2) HaCaT and 3) MCF7 cell lysate with Cytokeratin 5 antibody. Predicted molecular weight: 58-62 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P13647
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Cytokeratin 5 antibody is available for research use only.
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Description

Cytokeratin 5, also known as Keratin 5, KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Application Notes

Optimal dilution of the Cytokeratin 5 antibody should be determined by the researcher.

Immunogen

Amino acids 286-317 (KVELEAKVDALMDEINFMKMFFDAELSQMQTH-human) were used as the immunogen for the Cytokeratin 5 antibody.

Storage

After reconstitution, the Cytokeratin 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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