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Home >> Antibodies >> Cytokeratin 5 Antibody / KRT5

Cytokeratin 5 Antibody / KRT5 [clone KRT5/2080] (V7911)

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  Catalog No Formulation Size Price (USD)  
Image V7911-0.5ML Culture supernatant with 0.05% sodium azide 0.5 ml 519
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V7911-0.1ML Culture supernatant with 0.05% sodium azide 0.1 ml 229
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IHC staining of FFPE human basal cell carcinoma with Cytokeratin 5 antibody (clone KRT5/2080). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human basal cell carcinoma with Cytokeratin 5 antibody (clone KRT5/2080). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma with Cytokeratin 5 antibody (clone KRT5/2080). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Culture supernatant
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name KRT5/2080
Purity Culture supernatant
UniProt P13647
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This Cytokeratin 5 antibody is available for research use only.
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Description

This MAb recognizes a protein of 58kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium. Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express. Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5.

Application Notes

Optimal dilution of the Cytokeratin 5 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 318-491) was used as the immunogen for this Cytokeratin 5 antibody.

Storage

Store the Cytokeratin 5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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