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Home >> Antibodies >> Cytokeratin 18 Antibody

Cytokeratin 18 Antibody [clone CTKN18-1] (V7110)

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  Catalog No Formulation Size Price (USD)  
Image V7110-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7110-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7110SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7110IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 18 antibody (clone CTKN18-1, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 18 antibody (clone CTKN18-1, green) and Reddot nuclear stain (red).
IHC testing of FFPE human lung carcinoma and Cytokeratin 18 antibody (clone CTKN18-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 min, followed by cooling at RT for 20 min, prior to staining.
IHC testing of FFPE human thyroid carcinoma and Cytokeratin 18 antibody (clone CTKN18-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 min, followed by cooling at RT for 20 min, prior to staining.
IHC testing of FFPE human colon carcinoma and Cytokeratin 18 antibody (clone CTKN18-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 min, followed by cooling at RT for 20 min, prior to staining.
Western blot testing of HCT-116 cell lysate with Cytokeratin 18 antibody (clone CTKN18-1). Predicted molecular weight ~48 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CTKN18-1
Purity Protein G affinity chromatography
UniProt P05783
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Cytokeratin 18 antibody is available for research use only.
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Description

Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. [Wiki]

Application Notes

Titering of the Cytokeratin 18 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human KRT18 protein was used as the immunogen for the Cytokeratin 18 antibody.

Storage

Store the Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all Cytokeratin 18 antibodies

   2.   View all Keratin antibodies

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