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Home >> Antibodies >> cIAP1 Antibody / BIRC2

cIAP1 Antibody / BIRC2 (RQ4201)

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  Catalog No Formulation Size Price (USD)  
Image RQ4201 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) Jurkat, 3) MCF7, 4) COLO320, 5) U-87 MG, 6) A549, 7) rat thymus, 8) mouse thymus and 9) mouse testis lysate with cIAP1 antibody at 0.5ug/ml. Predicted molecular weight ~70 kDa.
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Western blot testing of human 1) HeLa, 2) Jurkat, 3) MCF7, 4) COLO320, 5) U-87 MG, 6) A549, 7) rat thymus, 8) mouse thymus and 9) mouse testis lysate with cIAP1 antibody at 0.5ug/ml. Predicted molecular weight ~70 kDa.
Flow cytometry testing of human U-2 OS cells with cIAP1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cIAP1 antibody.
IF/ICC staining of FFPE human U-2 OS cells with cIAP1 antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q13490
Applications Western Blot : 0.5-1ug/ml
Flow : 1-3ug/10^6 cells
Immunofluorescence/Immunocytochemistry (FFPE) : 2-4ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This cIAP1 antibody is available for research use only.
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Description

Baculoviral IAP repeat-containing protein 2 (also known as cIAP1) is a protein that in humans is encoded by the BIRC2 gene.The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the cIAP1 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids D320-T570 was used as the immunogen for the cIAP1 antibody.

Storage

After reconstitution, the cIAP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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