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Home >> Antibodies >> CDX2 Antibody

CDX2 Antibody [clone PCRP-CDX2-1A3] (V8501)

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  Catalog No Formulation Size Price (USD)  
Image V8501-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8501-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8501SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CDX2 antibody (clone PCRP-CDX2-1A3). These results demonstrate the foremost specificity of the PCRP-CDX2-1A3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CDX2 antibody (clone PCRP-CDX2-1A3). These results demonstrate the foremost specificity of the PCRP-CDX2-1A3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human colon carcinoma with CDX2 antibody (clone PCRP-CDX2-1A3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CDX2 antibody (clone PCRP-CDX2-1A3) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PCRP-CDX2-1A3
Purity Protein G affinity chromatography
UniProt Q99626
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This CDX2 antibody is available for research use only.
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Description

The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.

Application Notes

Optimal dilution of the CDX2 antibody should be determined by the researcher.

Immunogen

Recombinant human full-length CDX2 protein was used as the immunogen for the CDX2 antibody.

Storage

Store the CDX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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