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Home >> Antibodies >> Cdk2 Antibody

Cdk2 Antibody (R32030)

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Image R32030 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Flow cytometry testing of human U-2 OS cells with Cdk2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cdk2 antibody.
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Flow cytometry testing of human U-2 OS cells with Cdk2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cdk2 antibody.
Western blot testing of human 1) Jurkat and 2) HepG2 cell lysate with Cdk2 antibody. Predicted molecular weight ~33 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P24941
Localization Nuclear and cytoplasmic
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Cdk2 antibody is available for research use only.
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Description

CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein is highly homologous to p34 (CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34 (cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Application Notes

Optimal dilution of the Cdk2 antibody should be determined by the researcher.

Immunogen

Amino acids 81-298 of human Cdk2 were used as the immunogen for the Cdk2 antibody.

Storage

After reconstitution, the Cdk2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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