CDK12 Antibody / Cyclin-dependent kinase 12 (RQ6165)

Immunofluorescent staining of FFPE human A549 cells with CDK12 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human bladder urothelial carcinoma tissue with CDK12 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with CDK12 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate cancer tissue with CDK12 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) HeLa, 2) K562, 3) Jurkat and 4) HepG2 cell lysate with CDK12 antibody. Predicted molecular weight ~164 kDa but commonly observed at 164-205 kDa depending on the degree of protein modification.

Flow cytometry testing of human HepG2 cells with CDK12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDK12 antibody.