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Home >> Antibodies >> Cd86 Antibody

Cd86 Antibody (RQ6840)

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  Catalog No Formulation Size Price (USD)  
Image RQ6840 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of mouse ANA-1 cells with Cd86 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd86 antibody.
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Flow cytometry testing of mouse ANA-1 cells with Cd86 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd86 antibody.
IHC staining of FFPE rat spleen tissue with Cd86 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat lung and 2) mouse lung tissue lysate with Cd86 antibody. Predicted molecular weight ~38 kDa (unmodified), 45-70 kDa (glycosylated).
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P42082
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Cd86 antibody is available for research use only.
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Description

Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Application Notes

Optimal dilution of the Cd86 antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids T35-E221) was used as the immunogen for the Cd86 antibody.

Storage

After reconstitution, the Cd86 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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