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Home >> Antibodies >> CD74 Antibody / CLIP

CD74 Antibody / CLIP [clone CLIP/7192] (V5104)

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  Catalog No Formulation Size Price (USD)  
Image V5104-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5104-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5104SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human spleen tissue with CD74 antibody (clone CLIP/7192) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human spleen tissue with CD74 antibody (clone CLIP/7192) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD74 antibody (clone CLIP/7192) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji cell lysate with CD74 antibody (clone CLIP/7192). Expected molecular weight: 33-43 kDa.
SDS-PAGE analysis of purified, BSA-free CD74 antibody (clone CLIP/7192) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CLIP/7192
Purity Protein A/G affinity
UniProt P04233
Localization Cell surface, Paranuclear globular
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This CD74 antibody is available for research use only.
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Description

CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides.

Application Notes

Optimal dilution of the CD74 antibody should be determined by the researcher.

Immunogen

A recombinant fragment of human CD74 protein was used as the immunogen for the CD74 antibody.

Storage

Aliquot the CD74 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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