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Home >> Antibodies >> CD19 Antibody

CD19 Antibody [clone CVID3/429] (V2389)

  Catalog No Formulation Size Price (USD)  
Image V2389-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2389-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2389SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of human Raji cells with CD19 antibody (clone CVID3/429, green) and Reddot nuclear stain (red).
Flow cytometry testing of human Raji cells with CD19 antibody (clone CVID3/429); Red=isotype control, Blue= CD19 antibody.
Flow cytometry testing of lymphocyte-gated human PBM cells with CD19 antibody (clone CVID3/429); Gray=unstained cells, Violet= anti-CD19 antibody stained cells.
SDS-PAGE analysis of purified, BSA-free CD19 antibody (clone CVID3/429) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CVID3/429
Purity Protein G affinity chromatography
UniProt P15391
Localization Cell surface, cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Limitations This CD19 antibody is available for research use only.
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Description

CD19 is expressed on follicular dendritic cells and B cells. It is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. [Wiki]

Application Notes

Variations in protocols, secondaries and substrates may require the CD19 antibody to be titered up or down for optimal performance.

Immunogen

Human CD19 recombinant full length protein was used as immunogen for this CD19 antibody.

Storage

CD19 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

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