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Home >> Antibodies >> CD15 Antibody

CD15 Antibody [clone MY-1] (V8496)

  Catalog No Formulation Size Price (USD)  
Image V8496-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8496-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8496SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of human U937 cells with CD15 antibody (clone MY-1, green) and Reddot nuclear stain (red).
IHC staining of FFPE human Hodgkin's lymphoma with CD15 antibody (clone MY-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgM, kappa
Clone Name MY-1
Purity Protein G affinity chromatography
UniProt P22083
Localization Cell surface and granular paranuclear
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This CD15 antibody is available for research use only.
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Description

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Application Notes

Optimal dilution of the CD15 antibody should be determined by the researcher.

Immunogen

Purified neutrophils from normal human peripheral blood were used as the immunogen for the CD15 antibody.

Storage

Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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